METHODS: Ten untrained to recreationally active men (n=5) and women (n=5) completed a counterbalanced, crossover study. Four days of prolonged sitting without exercise (SIT) were compared to four days of prolonged sitting with a 1-hr bout of treadmill exercise (SIT+EX; 63.1±5.2% V̇O2max) on the evening of the fourth day. The following morning, participants completed a high fat/glucose tolerance test (HFGTT), during which plasma was collected over a 6-hr period and analyzed for triglycerides, glucose, and insulin. RESULTS: No differences between trials ( P > 0.05) were found in the overall plasma triglyceride, glucose, or insulin responses during the HFGTT. This lack of difference between trials comes with similarly low physical activity (~3,500-4,000 steps/day) on each day except for the 1-hr bout of exercise during SIT+EX the day before the HFGTT. - www.ncbi.nlm.nih.gov